Using the whole-cell patch-clamp technique
Calcium current is measured under whole-cell patch-clamp
conditions.
Cells are continuously superfused with bath solution
at room temperature. The conventional whole-cell patch-clamp
configuration was used to record membrane currents.
Composition of the bath solution
The composition of the bath solution is (mM):
NaCl 125; NaHCO3 25; KCl 5.4; CaCl2 1.8; MgCl2
1.0; NaH2PO4 1.2; D-glucose 5.5; CsCl 5; pH
7.4 when bubbled with 95% CO2 and 5% O2
mixture.
Composition of the pipette solution
The pipette solution is prepared in one batch prior to the
start of the experimental phase of the study and is stored in 0.5 mL
aliquots at approximately à °C until the day of use. The
composition of the pipette solution is (mM): CsCl 120; TEACl 20;
MgCl2 5.5; ethylene glycol-bis(ßaminoethyl ether)-N,N,Nî’¼/i>-tetraacetic
acid (EGTA) 5; ATP-Na2 5, HEPES 20; phosphocreatine 5 pH
7.2 with 1M CsOH.
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