ION CHANNEL ASSAYS
VENTRICULAR MYOCYTE CELL PAIRS
EFFECTS OF HEPTANOL ON JUNCTIONAL RESISTANCE MEASURED IN VENTRICULAR MYOCYTE CELL PAIRS
Effect of heptanol on junctional
conductance measured in isolated ventricular myocyte cell pairs.
shows the effects of the uncoupling agent heptanol on junctional
current amplitude. A repetitive step hyperpolarisation to -50
mV (0.2 Hz) is used to monitor the stability of the amplitude of IJ.
After 2 minutes control recording the perfusate is switched to one
containing heptanol (as denoted by the black bar).
which is a known uncoupler of cardiac gap junctions causes an
immediate and complete block of IJ amplitude. On
wash off of heptanol; there was complete recovery of IJ.